Problems, possible solutions

Possible Problems:

Solutions:

LC related

Defocusing effects;
Light scattering;
Adiabatic following of
polarization in chiral LCs;

Use small Δn LCs or scanning depth z so that Δnz/λ is of the order of unity or less;

Dye related

Gradients of dye concentration;
Inhomogeneous signal losses;
Nonlinear optical effects

Use small concentration of dye with high solvability and high quantum yield

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