The basic idea of the FastFCPM is that the scanning is performed by multiple laser beams simultaneously rather than by one beam as in a conventional confocal microscope. Our FastFCPM set up is based on the fast scanning CARV® system with a spinning dual Nipkow disk with 20,000 of pinholes and micro-lenses integrated with a Nikon microscope Eclipse E-600 POL base. Vertical refocusing is performed by a fast piezo z-stepper capable of scanning a 10 micron-thick sample by producing 10 frames in less than 5 ms. For repetitive processes, one can use only xy-scanning, refocusing it at different depth step-by step. We modified this instrument by adding a liquid-crystalline electrically driven polarization rotator to control the polarization of probing light. The capabilities of the Fast version of FCPM are expanded into the time domain of real-time scanning; Fast FCPM allows one for the study of the dynamic processes at the time scale of about 1ms.
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